Differential expression analysis of RNA–Seq data using DESeq2
نویسنده
چکیده
3 RNA–Seq data preprocessing 2 3.1 Creation of a sample metadata table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 3.2 Quality control commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.3 Alignment of reads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.4 Sorting and indexing of the alignment files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 3.5 Counting features with HTSeq . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 3.6 Creating a count table for DESeq2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 3.7 Add additional annotation information using biomaRt . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
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تاریخ انتشار 2014